Analytik Jena Specord 200, Specord 205, Specord 210, Specord 250 User manual (2022)

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Analytik Jena Specord 200, Specord 205, Specord 210, Specord 250 User manual (2)

SPECORD® PC 200 / 205 / 210 / 250

UV VIS Spectrophotometer

Analytik Jena Specord 200, Specord 205, Specord 210, Specord 250 User manual (3)

Analytik Jena AG

Kundendienst

Konrad-Zuse-Str. 1

07745 Jena

Phone:

Hotline:

+ 49 (0) 3641 77 74 07

Fax:

+ 49 (0) 3641 77 74 49

E-Mail:

service@analytik-jena.de

General information about Analytik Jena AG

on the internet: http://www.analytik-jena.de

Copyrights and Trademarks

Microsoft, Windows XP, Windows NT, Windows 2000, MS Excel are registered trademarks of Microsoft Corp.

Publication No.: 221:416.23

Edition – April 2005

Technical documentation made by:

Analytik Jena AG

This documentation describes the state of this product at the time of publishing. It need not necessarily agree with future versions of the product.

Subject to change!

© Copyright 2005 Analytik Jena AG

Analytik Jena Specord 200, Specord 205, Specord 210, Specord 250 User manual (4)

SPECORD®

Contents

Contents

1

Introduction .....................................................................

3

1.1

Intended use of the SPECORD.............................................................

3

1.2

Notes on the User's Manual..................................................................

4

2

Safety notes .....................................................................

5

2.1

Warning labels on SPECORD...............................................................

5

2.2

General safety regulations ....................................................................

5

3

Technical data of the spectrophotometers ...................

9

3.1

Optical data of SPECORD ....................................................................

9

3.2

General technical data of SPECORD .................................................

11

4

Device description and operating principle ................

13

4.1

Mechanical design of SPECORD .......................................................

13

4.2

Design of subassemblies ....................................................................

14

4.2.1

Light sources..................................................................................................

14

4.2.2

Spectrometer system and photometer section ...............................................

16

4.2.3

Sample compartment .....................................................................................

17

5

Installation and transport conditions ..........................

19

6

Installation and start-up................................................

21

6.1

Connectors and display elements.......................................................

21

6.2

Removing the transport lock ...............................................................

23

6.3

Connecting the SPECORD .................................................................

24

(Video) Analytik Jena UK Specord Plus Video

6.4

Start-up................................................................................................

25

6.5

Switching the device off ......................................................................

26

6.5.1

Switching the deuterium lamp off ...................................................................

26

6.5.2

Switching the SPECORD off ..........................................................................

26

7

Accessories ...................................................................

27

7.1

Standard cell holders ..........................................................................

27

7.2

Receptacle for cells with turbid samples.............................................

28

7.3

Additional accessories ........................................................................

29

8

Care, maintenance, lamp change.................................

31

8.1

Care and maintenance........................................................................

31

8.2

Cleaning ..............................................................................................

31

8.3

Changing lamps ..................................................................................

32

8.3.1

Note on the service life of lamps ....................................................................

32

8.3.2

Opening the flap of the lamp compartment ....................................................

32

8.3.3

Changing the deuterium lamp ........................................................................

35

8.3.4

Changing the halogen lamp ...........................................................................

37

8.4

Changing the desiccator .....................................................................

39

8.5

Changing fuses ...................................................................................

39

9

Disposal .........................................................................

41

10

Index to Illustrations .....................................................

43

11

Index to Overviews........................................................

43

Analytik Jena Specord 200, Specord 205, Specord 210, Specord 250 User manual (5)

SPECORD®

Introduction

Intended use of the SPECORD

1 Introduction

1.1Intended use of the SPECORD

The SPECORD® is a powerful, computer-controlled double-beam spectrophotometer for the measurement of the transmission, absorbance, reflection as well as the energy of liquid and solid samples. It is designed for measurements in the spectral range 190 – 1100 nm.

The double-beam spectrophotometers of the SPECORD® family provide different spectral resolutions:

SPECORD® 200

1; 2 and 4 nm

SPECORD® 205

1.4 nm

SPECORD® 210

0.5, 1,

2 and 4 nm

SPECORD® 250

0.5; 1;

2 and 4 nm, with premonochromator

The optical system without electromechanical modulator ensures true real-time operation, so that even variations in the absorbance of references can be compensated during the measurement.

The internal wavelength calibration with a holmium oxide filter provides a high wavelength accuracy and reproducibility.

The SPECORD® complies with the regulations of the DAB (German Pharmacopoeia) and international regulations, such as PhEur., BP, USP or JPXIII.

In combination with the powerful WinASPECT® software, the SPECORD® is particularly suitable for the use in routine laboratories with a high sample throughput, but also provides solutions for special applications, if used in combination with the diverse accessories available.

SPECORD® 200/205/210/250 User's Manual – Edition 04/2005

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Analytik Jena AG

Analytik Jena Specord 200, Specord 205, Specord 210, Specord 250 User manual (6)

(Video) Tutorial Praktikum Spektrofotometri

Introduction

SPECORD®

Notes on the User's Manual

1.2Notes on the User's Manual

This manual contains the description of the following devices

SPECORD® 200

SPECORD® 205

SPECORD® 210

SPECORD® 250

If the descriptions equally refer to all three models, only the collective term SPECORD® will be used. If a property of an individual model shall be particularly stressed, the full name of the model will be mentioned.

In this manual, the following symbols are used to refer you to warnings and notes:

Warning!

Warning messages alert you to a specific procedure or practice which, if not followed correctly, could cause personal injury.

Caution!

Caution messages refer you to procedures which, if not observed, could result in damage to the equipment.

Dangerous electric voltage!

Emission of UV radiation!

Disconnect power cable!

Note

This note must be followed to obtain correct measurement results.

For easier navigation within the manual, the manual uses the following system:

Chapters and illustrations are numbered consecutively.

Every illustration has its own caption.

Steps of operation are numbered.

Cross-references are marked by an arrow (e.g. →Intended use of the SPECORD p.3)

Page 4

SPECORD® 200/205/210/250 User's Manual – Edition 04/2005

Analytik Jena AG

Analytik Jena Specord 200, Specord 205, Specord 210, Specord 250 User manual (7)

SPECORD®

Safety notes

Warning labels on SPECORD

2 Safety notes

2.1Warning labels on SPECORD

Observe the warning labels on the SPECORD®!

The following warning labels have been affixed to the lamp housings of the SPECORD®:

Danger! UV radiation!

Do not look directly or indirectly via a mirror into the radiation emitted by the UV lamp.

The lamp radiation may cause conjunctivitis!

Danger! Hot surface!

When lamps are switched on, their covers heat up strongly. If you intend to change the lamps or open the lamp covers, switch the lamps off and allow for a sufficient cooldown time.

2.2General safety regulations

For your personal safety and for a trouble-free operation, carefully read this chapter before starting up the SPECORD®.

Observe all safety notes given in this manual and all messages and notes displayed by the control software on the screen.

In addition, observe the safety notes for system components of other manufacturers (e.g. PC, printer, autosampler) supplied together with the device.

In particular, you should also observe the safety notes given on the labels and the information on the use, storage and disposal of reagents, reaction cells and the notes on the package inserts of test kits.

Intended use!

The SPECORD®, including original accessories, may only be used for the applications described in this manual. The manufacturer cannot assume any liability for any other application, including that of individual modules or single parts. This also applies to all service or repair work that is not carried out by authorized service personnel. All warranty claims shall be forfeited.

Local safety regulations!

Observe the local safety regulations relevant to the operation of the device (e.g. laborsafety regulations, regulations for the prevention of and protection against accidents).

References to possible risks given in this manual do not replace the relevant laborsafety regulations that are to be followed.

Personnel!

The SPECORD® may only be operated by appropriately qualified and trained personnel.

For that, the knowledge of the information provided by this manual is indispensable and taken for granted.

SPECORD® 200/205/210/250 User's Manual – Edition 04/2005

Page 5

Analytik Jena AG

Analytik Jena Specord 200, Specord 205, Specord 210, Specord 250 User manual (8)

Safety notes

SPECORD®

General safety regulations

Emergency stop!

In the case of emergency, disconnect the SPECORD® and its components from line power by removing the power plug from the power outlet.

Electric shocks!

The SPECORD® is electrically powered. At several parts inside the system, extremely hazardous electrical voltages are accessible!

To ensure Protection Class I (protective earth connection) of the device, connect the power plug of the device only to a power outlet with protective earth conductor. The protective effect must not be undone by the use of an extension cable without protective earth conductor.

Before opening the device or removing any device covers, disconnect the power cable!

Before connecting the SPECORD® to the power outlet, make sure the line voltage supplied agrees with the operating voltage specified on the rating plate at the rear panel of the device. Operation of the device with any other operating voltage than that specified may result in the destruction of the device.

Only use fuses of the specified type.

Do not operate the device in explosion-risk rooms!

UV radiation!

Never look directly or indirectly, e.g. via a mirror, into the radiation emitted by the lamp to avoid the risk of getting conjunctivitis!

Therefore, switch off the lamp for lamp replacement!

Lamp damage!

Do not grasp functioning halogen and deuterium lamps on their glass bulbs. Contamination on the bulb affects the radiation properties of the lamps. Especially protect the beam-exit window of the deuterium lamp.

Make sure to remove the protective cap from the halogen bulb after having inserted it to avoid any risk of fire!

Non-observance will result in damage of the lamp.

Accumulation of heat

Heat accumulation might result in overheating and faults on the device.

Make sure not to cover the ventilation slots of the SPECORD®!

Before you switch on the SPECORD®, remove its dust cover!

Handling liquids

Take care to ensure that no liquids can enter the SPECORD® to avoid any damage to the device.

Risk of corrosion

Do not set up the device in the vicinity of aggressive vapors, e.g. strongly corrosive acid or caustic vapors! The vapors might corrode the connections, mechanical and optical components of the device.

Avoid exposing the sample compartment to strongly corrosive substances! For the analysis of such samples, use vapor-tight stoppered cells.

After the measurement, do not leave the samples unnecessarily long in the sample compartment of the device!

Page 6

SPECORD® 200/205/210/250 User's Manual – Edition 04/2005

Analytik Jena AG

Analytik Jena Specord 200, Specord 205, Specord 210, Specord 250 User manual (9)

SPECORD®

Safety notes

General safety regulations

The SPECORD® was produced and tested in compliance with the following standards and regulations:

DIN EN 61010-1 (IEC 1010-1)

73/23/EC

89/336/EC.

The SPECORD® meets the requirements as per Protection Type IP 20.

SPECORD® 200/205/210/250 User's Manual – Edition 04/2005

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Analytik Jena AG

Analytik Jena Specord 200, Specord 205, Specord 210, Specord 250 User manual (10)

SPECORD®

Technical data of the spectrophotometers

Optical data of SPECORD

3

Technical data of the spectrophotometers

3.1

Optical data of SPECORD

Technical Data

SPECORD® 200 / 205 /210

Principle

Double-beam spectrophotometer with two large-area

photodiodes

Optics

Monochromator with holographic concave grating

Light split into two beams by a beam-splitting plate

Wavelength range

190-1100nm

Photometric display range

-8A ... 8A

Photometric measuring range

-3A ... 3A

Wavelength accuracy

≤ ± 0.5nm

(with holmium oxide filter)*

Wavelength reproducibility

≤ ± 0.05nm

(with holmium oxide filter)*

Photometric accuracy VIS

≤ ± 0.005

at 546nm with Hellma F4

neutral-density filter

Photometric accuracy UV

≤ ± 0.01

with potassium dichromate as per

Ph.Eur.

Stray light at 200nm

(KCl Merck 1.08164.0001)

≤ 0.5%T

Stray light at 220nm (NaI)

≤ 0.05%T

Stray light at 240nm (NaI)

≤ 0.05%

Stray light at 340nm (NaNO2)

≤ 0.05%T

Baseline noise at 500nm

≤ 0.00015 (RMS)

Long-term stability at 500nm

≤ ± 0.001

Scanning speed

up to 6000nm/min

* allowing for tolerances of used standard

SPECORD® 200/205/210/250 User's Manual – Edition 04/2005

Page 9

Analytik Jena AG

Analytik Jena Specord 200, Specord 205, Specord 210, Specord 250 User manual (11)

Technical data of the spectrophotometers

SPECORD®

Optical data of SPECORD

Technical Data

SPECORD® 250

Optical principle

Double-beam spectrophotometer with two large-area

photodiodes

Optics

Premonochromator and monochromator with holographic

concave grating

Light split into two beams by a beam-splitting plate

Wavelength range

190-1100nm

Photometric display range

-8A … 8A

Photometric measuring range

-4A … 4A

Wavelength accuracy

≤ ± 0.5nm

(with holmium oxide filter)*

Wavelength reproducibility

≤ ± 0.05nm

(with holmium oxide filter)*

Photometric accuracy VIS

≤ ± 0.005

at 546nm with Hellma F4

neutral-density filter

Photometric accuracy UV

≤ ± 0.01

with potassium dichromate as per

Ph.Eur.

Stray light at 200nm

(KCl Merck 1.08164.0001)

≤ 0.2%T

Stray light at 220nm (NaI)

≤ 0.008%T

Stray light at 240nm (NaI)

≤ 0.008%

Stray light at 340nm (NaNO2)

≤ 0.008%T

Baseline noise at 500nm

≤ 0.00015 (RMS)

Long-term stability at 500nm

≤ ± 0.001

Scanning speed

up to 6000nm/min

* allowing for tolerances of used standards

SPECORD® 200

SPECORD® 205

SPECORD® 210

SPECORD® 250

Spectral

1; 2; 4 nm variable

1.4 nm

0.5; 1; 2; 4 nm

0.5; 1; 2; 4 nm

bandwidth

Toluene / hexane

> 2.1

> 1.7

> 2.1

> 2.1

resolution

Overview 3-1 Technical data of SPECORD® 200

Page 10

SPECORD® 200/205/210/250 User's Manual – Edition 04/2005

Analytik Jena AG

Analytik Jena Specord 200, Specord 205, Specord 210, Specord 250 User manual (12)

SPECORD®

Technical data of the spectrophotometers

General technical data of SPECORD

3.2General technical data of SPECORD

Weight

23.5 kg

Footprint (W x D)

ca. 1200 x 750 mm2 mit PC

Dimensions (W x H x D)

680 x 240 x 620 mm3

Line voltage

230

VAC ±10 % or

115

VAC ±10 % (switchable by service)

Line frequency

47 ... 63 Hz

Power consumption

200

VA

Fuses:

230

V~: T 3,15 AH/250V, Type 19181 (Wickmann)

Line fuses

115

V~: T 3,15 AH/250V, Type 19181 (Wickmann)

Radio noise suppression

as per EN 55011 Class A

Noise immunity

as per DIN EN 50082-1

Protection Type

IP 20

Data interfaces

2 x RS-232 for accessories

1 x USB port for the connection of an external PC

1 x RS–232 for the connection of an external PC

PC requirements for software:

Pentium processor 133 MHz, 16 MB RAM,

WinASPECT

Windows 2000 or higher

Overview 3-2 General technical data of SPECORD®

SPECORD® 200/205/210/250 User's Manual – Edition 04/2005

Page 11

Analytik Jena AG

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